Journal: STAR Protocols

Article Title: Protocol for germ lineage differentiation of primed human pluripotent stem cells using chemically defined, nutrient-balanced media

doi: 10.1016/j.xpro.2022.101568

Figure Lengend Snippet:

Article Snippet: Timing: 30 min hPSCs will be maintained in culture and differentiated in feeder-free conditions on tissue culture-treated polystyrene microplates coated with Corning Matrigel® basement membrane matrix.

Techniques: Recombinant, Cell Culture, Suction Filtration

Journal: Frontiers in Microbiology

Article Title: Hyperosmotic Infusion and Oxidized Surfaces Are Essential for Biofilm Formation of Staphylococcus capitis From the Neonatal Intensive Care Unit

doi: 10.3389/fmicb.2020.00920

Figure Lengend Snippet: (A) Biofilm formation of 17 clinical S. capitis isolates on tissue culture polystyrene (TCPS) surfaces under different environmental conditions. Microplates were seeded with bacteria in the indicated media, including Tryptic soya broth (TSB) only, TSB + 1% glucose, TSB + 4% ethanol, and TSB + 4% NaCl. Biofilm formation was monitored using crystal violet staining. Error bars represent standard errors of the means (SEM). (B) The indicated isolates were grown in TSB or in TSB + 4% NaCl for 5.5 h and the expression of icaADBC and icaR at these two conditions were analyzed and compared by quantitative reverse transcription polymerase chain reaction (RT-PCR). Isolates 8, 11, 18, 19, and 21 were also examined and showed similar results as isolates 6. Error bars represent SEM.

Article Snippet: Nine commercially available polystyrene microplates with different surface characteristics were tested, including DNA-BIND (Corning), Carbo-BIND (Corning), Universal-BIND (Corning), Not Treated (Corning), CellBIND (Corning), Ultra-Low-Attachment (Corning), Immobilizer (Corning), Tissue Culture Polystyrene (TCPS) from NUNC, and TCPS from BD Falcon.

Techniques: Bacteria, Staining, Expressing, Reverse Transcription, Polymerase Chain Reaction, Reverse Transcription Polymerase Chain Reaction

Journal: Frontiers in Microbiology

Article Title: Hyperosmotic Infusion and Oxidized Surfaces Are Essential for Biofilm Formation of Staphylococcus capitis From the Neonatal Intensive Care Unit

doi: 10.3389/fmicb.2020.00920

Figure Lengend Snippet: Characterization of biofilm development of S. capitis isolates 6 and 19 on tissue culture polystyrene (TCPS) and Not Treated surfaces. (A) Biofilm development on the Not Treat surface in the first 6 h monitored by crystal violet staining and light microscopy. S. capitis isolates 6 and 19 failed to network their microcolony structures and form biofilms on Not Treated surfaces. S. epidermidis RP62a, used as a control, formed typical macrocolonies on the Not Treated surface at 6 h. Scale bar = 20 μm. (B) Biofilm matrix of S. capitis isolates 6 and 9 was extracted, and the soluble extract was incubated with either TCPS or Not Treated surfaces, followed by wheat germ agglutinin (WGA) staining and fluorescence microscopy. Scale bar = 100 μm.

Article Snippet: Nine commercially available polystyrene microplates with different surface characteristics were tested, including DNA-BIND (Corning), Carbo-BIND (Corning), Universal-BIND (Corning), Not Treated (Corning), CellBIND (Corning), Ultra-Low-Attachment (Corning), Immobilizer (Corning), Tissue Culture Polystyrene (TCPS) from NUNC, and TCPS from BD Falcon.

Techniques: Staining, Light Microscopy, Control, Incubation, Fluorescence, Microscopy